$249.0 Buy It Now or Best Offer
free,30-Day Returns
Seller Store docstill299n
(1626) 100.0%,
Location: Gainesville, Florida
Ships to: US,
Item: 154479311690
Return shipping will be paid by:Buyer
All returns accepted:Returns Accepted
Item must be returned within:30 Days
Refund will be given as:Money Back
480:480
Modified Item:No
Country/Region of Manufacture:United States
Custom Bundle:No
Number of Wells:48
PerkinElmer:PerkinElmer
Intended Use/Discipline:Biological Laboratory
Number of Blocks:1
Perkin Elmer DNA Thermal Cycler 480 – TESTED – Heats & Cools!!! Clean! 120 VAC. Excellent condition. Clean! Tested – Functions as it should – Heats AND Cools quickly and accurately. This is Important! Don’t buy an untested unit that has lost its Freon and can no longer cool. Has a small gouge on lower right of vinyl cover for display. Not in line of sight. Not a tear – just a scratch. Does not affect operation. Has a piece of yellow tape with note to remind you to put oil on your samples. Used condition – has some light scratches here and there (like on the plastic sample cover – visible in photos). Display is clear and bright. The fuzziness in some of the photos is because the camera catches a reflection from the cover. Look at photo of the rear – the cooling fins inside unit are almost as Clean as when New! This is very important for efficient cooling operation! 48 well unit. Power cable included. Guaranteed! 30-day return (buyer pays return shipping). Description and Instructions from web: The Perkin-Elmer/ Cetus DNA Thermal Cycler automates the polymerase chain reaction (PCR) technique for amplifying specific DNA sequences. A microprocessor allows you to create programs consisting of time and temperature reaction parameters for individual PCR reactions. These programs can be stored in files which may be modified further for different reactions. In addition, files may be linked together, allowing for more complex programs or “soaking” at 4 degrees C after completion of cycling when reactions are performed overnight. Files belong to one of two categories, incubation files or cycle files. Files 1 thru 4 are default files which have been programmed with typical parameters. These files can carry out the basic functions of the PCR machine, and they serve as template files which can be modified to program a new PCR reaction or to create user specific files for storage. File Categories for Default Files Cycle files: Incubations files: Soak (#1) – maintains one temperature until file Thermo-Cycle (#3) – a PCR amplification termination, may be linked to end of cycle file. Step-Cycle(#4) – also a PCR amplification Time Delay (#2) – incubates samples at a single program. temperature prior to running a cycle file. These simplified instructions for using the Perkin-Elmer/Cetus PCR machine will include how to access a file, edit the file, run and/ or save the edited file. For a more detailed description of the various default files and programs, refer to the Perkin-Elmer/Cetus operating instructions manual. It is strongly recommended that you read these instructions and precautions in their entirety before using the thermal cycler. Operating the DNA Thermal Cycler 1. Turn the power switch to ON on the front of the machine. The cycler will then display the opening prompt: “THERMO-CYCLE FILE” STEP to edit file #3 2. Access the default Thermo-Cycle file #3 from the opening prompt by pressing the STEP key. (Other files are accessed by pressing FILE, press the number of the file you want, then press ENTER) 3. Edit the default Thermo-Cycle file #3 to conform to your desired reaction parameters as follows. When you press the STEP key, the display will take you through the parameters you must answer to modify the file. 4. The message or information on the display is what the instrument is prompting for acceptance or change, and the parameter being prompted is underscored by the cursor. Modification of the default Thermo-Cycle file #3 is a simple way to program a reaction and will be used to explain the machine’s operation here. When editing file #3, it is easiest to think of editing segments in pairs. The odd segments (1,3,5) create thermal transitions or ramping of the temperature block to the desired temperature, and the even segments (2, 4, 6) create the thermal plateaus for each of the the three basic steps of the PCR reaction (denaturing, annealing, polymerization). Thus, segments 1 and 2 are a pair, represent ramping of the temperature block to the denaturing temperature (seg. 1) and holding the denaturing temperature for the desired time interval (seg. 2), etc. Entering time zero in segment 7 ends the file. As you press STEP thru the default file Thermo-Cycle file #3, you will edit the parameters of each segment to the ones you desire as detailed in the example below. Example: a PCR reaction with the following reaction conditions: 1. Denature 94 degrees C 1 minute 10 seconds 2. Anneal 55 degrees C 2 minutes 3. Polymerize 72 degrees C 2 minutes 30 seconds 35 cycles To edit the first segment: Press STEP to access segment 1. The following will appear on the display: “Seg 1 Target Temp” (0-100) 94C with cursor prompt This is a thermal transition segment. You can type the temperature you wish to reach for the denaturing step by modifying the value indicated by the cursor. In this case, simply press ENTER to accept the default temperature of 94 degrees C. The time parameter now appears on the display: “Seg 1 Segment Time” (0-59) O min 1sec Because you want the transition to occur as quickly as possible, accept the default values of 0 min 1 seconds by pressing ENTER twice. To edit the second segment: The second segment is the thermal plateau for the denaturing segment of the reaction. After pressing ENTER as above, the following appears on the display: “Seg 2 Target Temp” (0-100) 94C Press ENTER again to accept the default temperature of 94 degrees C for denaturing. The following now appears on the display: “Seg 2 Segment Time” (0-999) 1 min 0 sec Press ENTER to accept the 1 minute value and the cursor moves to prompt the seconds parameter. Press 1, 0, and ENTER to enter a value of 10 seconds. The value 10 sec appears on the display, and when you press ENTER, the display moves to segment 3. You are now ready to edit segment 3. Edit segments 3 to 6 with the following parameters: * Segment 3 Temp: 55 degrees C Time: 0 min 1 sec * Segment 4 Temp: 55 degrees C Time: 2 min * Segment 5 Temp: 72 degrees C Time: 0 min 1 sec * Segment 6 Temp: 72 degrees C Time: 2 min 30 sec When you have finished editing 6 segments, you have created three thermal transition/plateau pairs. Segment 7 is preset to end the file. Accept the zeros in segment 7 and you have terminated the instructions in your file. Choose the default NO when prompted for Auto Segment Extension by pressing ENTER. The follwing appears on the screen: “Cycle Count” (1-99) 25 Press 3, 5, and ENTER to indicate 35 cycles. Now the following appears on the display: “Link to stored file” (0-99, 0=SHUT-OFF) You may now link the file to an already existing file, such as a stored soak file at 4 degrees C (if for instance the PCR machine will run overnight and you wish the temperature block to go to 4 degrees C after completion of cycling) or you may instruct the machine to shut off. Press the number of the file and ENTER to indicate a file to be linked to the end of your cycle file, or press 0 and ENTER to Shut-Off. The following now appears on the display: “End Of File” RUN-STORE-PRINT If you wish to store this file for future use, press NO to move the cursor to the “Store” choice and press ENTER. You will then be prompted to enter a user number and a file number under which to store the file for later recall. To run the newly modified file, press ENTER and then START. The parameters in the default file will return to their original “default” values after the reaction is run, ready to be modified again for subsequent reactions. Methods for linking files to create more complex reactions, overwriting files, using the printer and additional information may be found in the accompanying Perkin-Elmer/Cetus instruction manual. Note: Due to recent price increases from all carriers, listings with “Free Shipping” will now have a small shipping fee added for West Coast destinations. Sorry, but the alternative would be to raise purchase price for everyone. All items ship from Gainesville, FL.
Frequently Asked Questions About Perkin Elmer DNA Thermal Cycler 480 – TESTED – Heats & Cools!!! Clean! 120 VAC in My Website
www.nayeskafitness.com is the best online shopping platform where you can buy Perkin Elmer DNA Thermal Cycler 480 – TESTED – Heats & Cools!!! Clean! 120 VAC from renowned brand(s). www.nayeskafitness.com delivers the most unique and largest selection of products from across the world especially from the US, UK and India at best prices and the fastest delivery time.
What are the best-selling Perkin Elmer DNA Thermal Cycler 480 – TESTED – Heats & Cools!!! Clean! 120 VAC on www.nayeskafitness.com?
www.nayeskafitness.com helps you to shop online and delivers Guess to your doorstep. The best-selling Guess on www.nayeskafitness.com are: Guess Logo Faux Fur Shoulder Bag Black Multi Gold Guess Bag Handbag Shopper Shoulder Bag Ginevra Society Tote Rosewood New Guess Women’s Clelia Mini Crossbody Handbag Guess Iwona Women’s Handbag GUESS Women’s La Femme Handbag Slate Embossed Fold-Over Flap Shoulder Bag Charms Guess Hartley Cold Shoulder Top in Leopard Print Size S New With Tags Guess Women’s 57mm Biege Sunglasses GF0427-27W GUESS Ophelia Rose Floral Satchel Handbag Purse Handles / Crossbody Strap Guess Downtown Cool Convertible Mini Crossbody Bag Pink Chain Strap Lock Charm FRIENDS WITH YOU x GUESS (2021) Official Collab Embroidered Boxy T-Shirt Large Dark Gray Black Patent Leather GUESS Purse Heart Handles Crystal Rhinestone Y2K Guess Handbag, Los Angeles, New Guess TShirt Vtg Y2K Sparkly Punk Rock Glam Tee Purple Semi Sheer Tattoo Art L Guess Womens Loren Blue Ruffled Midi Formal Cocktail and Party Dress S BHFO 3929 Free Ship USA Chic Handbag GUESS SATCHEL Havana Straw Ladies Tangerine Bag Love Guess Women’s Lace-Trim A-Line Dress Size 14 Guess Womens Sleeveless Floral Print Dress Size 6 MSRP $108.00 Guess Factory Gradient Smoke Rectangular Men’s Sunglasses GF5107 01B 54 GUESS Cross Body Satchel With Top Handles Brown Tan Logo With Silver Accents GUESS by MARCIANO Golden Bronze Mini Clear 12″ Tote Y2K Vintage NEW Guess Womens White Viper Girl Crewneck Tee Graphic T-Shirt Top XS BHFO 9566 Guess Embossed Crocodile Alligator Tan Bag Large Purse Handbag Guess Womens SX821478 Black Monogram Leather Chain Strap Phone Crossbody Bag Womens Leather Bags GUESS VINTAGE handbag Shoulder bag Metallic Purple Leather GUESS Silver Studded and Rhinestones Clutch Purse Small 10” X 6” Missing Strap Guess Dream Tote Shoulder Bag in Brown GUESS MONOGRAM GOLD METALLIC Y2K SHOULDER HANDBAG Guess Dress NWT Guess Cactus Flower Ombre Maxi Dress Size XS Extra Small Snakeskin High Low Guess Women’s Noelle Elite Tote Handbag Guess LA Blouse Gold Chain Collar Purple Long Sleeve Top Gisela Large Logo Shirt Guess Naya Tote & Wristlet in Stone Multi NEW W/TAGS Guess Women’s Purse 8.5×6 X 4 In Wide Pre Owned Barely Used Womens Size XS Extra Small Black Short Sleeve Shirt / Top by Guess ~ Silver NWT New GUESS Black Tee Gothic Crown Logo Graphic Print Slim Fitted Sz M GUESS 220089 Authentic Men Leather Billfold Wallet with Valet ~ BLACK Guess Handbag-Shoulderbag Black Bowling Style Zip Top (ANGOLA – Ret.$70) NWT-NEW Guess Womens Blk Leather Clutch/Shoulder Bag/gold Chain Pre-owned NEW Guess Crossbody Handbag New ladies crossbody bag Guess shoulder bag handbag trend fashion women’s bag Guess Y2K Vintage Crossbody Mini Bag Rhinestone Heart Chain GUESS small gun metal gray faux leather satchel, purse, bag, handbag. GUESS Women’s Size Medium Shoulder Bag Light Brown Tan Vachetta GUESS Women’s Top Lace Neckline Stretch Pullover White Short Sleeve Size Small “G By Guess” Black Faux Leather Women’s Handbag, Pre-Owned (g) Purse New GUESS Los Angeles Women’s Short Sleeve T-Shirt X-SMALL Gray w/White, Pink Guess Womens Dark Gray Monogram Shoulder Strap Backpack Guess Grey/Black Women’s Shoulder Bag With Leather Strap NEW GUESS Logo Black White Nylon Gym Weekend Travel Duffle Bag Handbag Guess Women Evan Blush Quilt Faux Leather Crossbody Mini Backpack NEW FLAWS Guess Y2K Faux Alligator Leather Handbag Purse Pink Satin Liner Rhinestone Guess Seductive Homme Noir by Guess 3.4 oz EDT Cologne for Men New In Box Women’s Guess Leather Brown Handbag GUESS Purse Handbag Red Zip Double Handle Leather EUC Vintage Guess Womens Medium Sleeveless Top 90s Minimalist Retro Office Sheer USA Women’s Y2K Guess Bag NWOT GUESS Womens Lace Sheer Intimates Bodysuit Guess Shawna Cali Cognac Multi-Color Faux Leather Crossbody/Satchel – VG719005 G By Guess Clutch Bag Black Faux Leather Flap Envelope Zip Chain Guess los angeles size medium red/black short sleeve scoop neck tshirt Guess Slim Flapped Crossbody Bag Black Faux Leather Purse W/Logo Panel Guess Women’s Pink Sheer Tshirt Size Medium Y2K Guess Small Black Handbag with Silver Hardware Guess Women’s Purse Hand Bag Shoulder Bag Beige Faux Snake Print Small Pre-Owned GUESS PENN MAR MINI Crossbody With COIN Purse ; Women Shoulder Bag-BLACK 50 Cent Guess Who’s Back New Sealed Official Debut Mixtape CD Very Rare Womens Guess Betty Boop Crop Top Guess SG882798 Unisex Large Coal/Black Allover Logo Print Insignia Backpack Guess Vintage Lavender White Snake Skin Style Baguette Handbag Purse Guess Shirt Womens Medium V-Neck Classic Embossed Logo Short Sleeve Ladies NEW GUESS Los Angeles Women’s Short Sleeve T-Shirt Beige Size Small GUESS Women’s Red Crossbody Bag Size 10×8 Guess Handbag + Cosmetic Pouch 2pc Set Floral Kiss Me Pink Magnet Closure Tote Guess Los Angeles Mini Backpack LARGE RED GUESS ? PATENT FAUX LEATHER TWO HANDLED HAND BAG CLASSIC G GUESS WOMEN’S CRYSTAL RHINESTONE LOGO FULL ZIP FRONT TOP STRETCHY M NWT Guess Women’s 61mm Rose Gold Sunglasses GF0408-28F Guess Women’s 99mm Brown Sunglasses GF0400-32B Guess Womens Top Size M Red Solid Blouse Short Sleeve Cotton Shirt Casual NWT GUESS Vegan Leather RARE “Coast Babies” Camel Tan Mini Tote Purse Bag VTG GUESS Satin Purse 90s Style Lilac Shoulder Grommet Bag Pre-Owned GUESS DARE by Guess 3.4/3.3 OZ / 100 ML EDT SPRAY For Women Genuine Guess Black Satchel Guess Shoulder Handbag Purse PU Vegan Leather Stud Accented G Guess Semi Sheer Top Chateau Rose Sweetheart Keyhole Back Floral Top Size XS Sexy Skin Sweet Sugar by Guess for Women – 8.4 oz Fragrance Mist Guess Los Angeles Shirt Women Size XS Pearl Snap Long Sleeve Floral Cottagecore NWOT Black Guess Women’s Pink Floral Leather Crossbody Handbag w/ Coin Purse Guess Hand Bag Woman’s Red Mary Poppins Bag W/Separate Lower Zipper Compartment Vintage Y2K GUESS Handbag Monogram Shoulder Bag Canvas Suede 90s Logo Bling Guess Eco Jules Bodysuit Size L GUESS Womens Green Woven Tie Shirred Buttoned Sleeveless Scoop Neck Peplum Top L GUESS Satchel/Handle Bag medium perfect condition pink white interior Guess Whos Coming to Dinner – GOOD GUESS Black Wristlet Bag Clutch Purse Logo Wallet Black Guess Shoulder Bag Guess Cream Embroidered Top off the Shoulders UK8 XS Vintage GUESS USA Brown Leather Cross Body Bag – Hipster Purse Camera NEW Guess Dusty Mauve Cuba Shoulder Bag Tote 10″H x 15.5″W x 6″D Guess Gold Eau de Parfum Spray 50 ml. 1.7oz vintage Mens Pullover Fleece Hoodie